|
KMID : 1007520190280010191
|
|
Food Science and Biotechnology
2019 Volume.28 No. 1 p.191 ~ p.200
|
|
|
Acer okamotoanum protects SH-SY5Y neuronal cells against hydrogen peroxide-induced oxidative stress
|
|
Kim Ji-Hyun
Lee Sang-Hyun
Cho Eun-Ju
|
|
|
Abstract
|
|
|
|
Oxidative stress by over-production of reactive oxygen species (ROS) in brain is widely known as a cause of neurodegenerative disease. We investigated protective effects of Acer okamotoanum against oxidative stress by hydrogen peroxide (H2O2) in SH-SY5Y neuronal cells. Acer okamotoanum reduced ROS production and lactate dehydrogenase release in H2O2-induced SH-SY5Y cells, resulting in elevation of cell viability. To elucidate protective mechanisms, we measured inflammation and apoptosis-associated protein expressions. Treatment with A. okamotoanum dose-dependently decreased pro-inflammatory proteins such as inducible nitric oxide synthase and cyclooxygenase-2. Treatment with A. okamotoanum showed down-regulation of pro-apoptosis genes such as cleaved caspase-3, cleaved caspase-9, and Bax, and up-regulation of anti-apoptosis protein including Bcl-2, in H2O2-induced SH-SY5Y cells. We demonstrated potential anti-inflammatory and anti-apoptotic effect of A. okamotoanum in H2O2-induced SH-SY5Y cells. These results suggest that A. okamotoanum may possess neuroprotective potential, but further study is necessary to elucidate its pharmacological effects in neurodegenerative diseases.
|
|
|
KEYWORD
|
|
|
Acer okamotoanum, Apoptosis, Hydrogen peroxide, Inflammation, Oxidative stress
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
 
|